Part:BBa_K4417014:Design

pCT5c backbone

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 2009
  Illegal EcoRI site found at 5065
  Illegal XbaI site found at 5520
  Illegal SpeI site found at 5961
  Illegal PstI site found at 1257
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 2009
  Illegal EcoRI site found at 5065
  Illegal NheI site found at 5296
  Illegal SpeI site found at 5961
  Illegal PstI site found at 1257
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 2009
  Illegal EcoRI site found at 5065
  Illegal BamHI site found at 6867
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 2009
  Illegal EcoRI site found at 5065
  Illegal XbaI site found at 5520
  Illegal SpeI site found at 5961
  Illegal PstI site found at 1257
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 2009
  Illegal EcoRI site found at 5065
  Illegal XbaI site found at 5520
  Illegal SpeI site found at 5961
  Illegal PstI site found at 1257
  Illegal NgoMIV site found at 20
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal SapI site found at 6873
  Illegal SapI.rc site found at 5
  

Design Notes

This part was digested from the urease master plasmid (BBa_K4417017).

Source

The original plasmid was created by Claudia Schmidt-Dannert lab.

References

1. Development of a synthetic cumate-inducible gene expression system for Bacillus. Seo SO, Schmidt-Dannert C. Appl Microbiol Biotechnol. 2018 Nov 3. pii: 10.1007/s00253-018-9485-4. doi: 10.1007/s00253-018-9485-4. 10.1007/s00253-018-9485-4 PubMed 30392122